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The In Vivo Imaging Community
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The In Vivo Imaging Page provides a Discussion Forum and Links to information about the discipline of "In Vivo" Molecular Imaging with an emphasis on Pre-clinical Animal Models, not excluding Translational Imaging. Techniques covered are: Optical Imaging, Molecular Imaging, Fluorescence Imaging, Bioluminescence Imaging, MRI, PET, SPECT, CT, Ultrasound and Intravital Microscopy.
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In Vivo Imaging - White Papers
In Vivo Optical Imaging Reporters
There is a plethora of choice in reporter tags for non-invasive in vivo optical imaging (fluorescence and bioluminescence) that are currently available from various commercial suppliers. This generates tremendous flexibility on one hand, but dilemma of choice on the other. This manuscript reviews the determining factors and will facilitate decision making using an interactive flow chart. Read more
D-Luciferin: in vivo imaging methods
D-luciferin [(S)-2-(6′-hydroxy-2′-benzothiazolyl)thiazoline-4-carboxylic acid] is the substrate of the North American firefly Photinus Pyralis luciferase and Clickbeetle Red and Clickbeetle Green luciferase. Luciferin is a low molecular weight (318.41 g/mole) organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is a small molecule which freely diffuses across membranes. When luciferin is injected in vivo Read more
Mouse Imaging: mouse strains for optical imaging
Laboratory mice are the primary model in preclinical research. This review discusses the characteristics of mouse strains of interest for in vivo optical mouse imaging in general and for cancer models. Read more
Cell Culture Techniques for In Vivo Grafting
Mouse Microbubble Imaging – contrast enhanced ultrasound, gene therapy and drug delivery
Microbubbles for in vivo ultrasound mouse imaging are encapsulated, inert gas filled colloidal particles. Upon intravenous injection, they are used as ultrasound contrast agents. Read more
In Vivo imaging - In the News
Study finds how nanoparticles indirectly damage DNA in cells
British researchers who built a multi-layer "barrier" of human cells to replicate the function of specialized protective tissues in the body found that signals transmitted by nanoparticles through the protective barrier indirectly damage DNA inside cells. The study results "expand significantly the hurdles that any theoretical nano-safety assessment would need to clear," an expert said. The discovered mechanism, however, also opens up the possibility of being able to deliver treatments across barriers without actually crossing them, another expert suggested.
Canary Funds Early Cancer Center at Stanford
NEW YORK (GenomeWeb News) – Stanford University will use a $15 million commitment from the Canary Foundation, along with $5 million of its own funds, to start a cancer research center focused on using proteomics and imaging techniques to find ways to identify cancer earlier.
Preclinical Drug Development Must Consider the Impact on Metastasis
Drs. Steeg et al., President and members of the "Metastasis Research Society " propose that preclinical anticancer drug development be required to show efficacy in at least one metastasis model, preferably incorporating metastasis from an orthotopic site. Robust models are available with relevant histology and Imaging for quantification.
Clin Cancer Res 2009; 15 (14) July 15, 2009
Infrared-fluorescent proteins for whole body imaging
2008 Nobel-Prize winner Roger Tsien, Ph.D., has engineered infrared-fluorescent proteins (IFPs) that are are suitable for whole-body imaging in small animals.
The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008 (Jove video)
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May 26-29, 2010 - Warsaw, Poland
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Recent Publications : Pubmed - Luciferase In Vivo Imaging
- Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring.
- miR-29 is a key regulator of collagen expression in systemic sclerosis.
- Akt is a direct target for myricetin to inhibit cell transformation.
- Oncostatin M promotes bone formation independently of resorption when signaling through leukemia inhibitory factor receptor in mice.
- In vivo detection of extrapancreatic insulin gene expression in diabetic mice by bioluminescence imaging.
PubMed: Fluorescence In Vivo Imaging
- Rapid pharmacokinetic and biodistribution studies using cholorotoxin-conjugated iron oxide nanoparticles: a novel non-radioactive method.
- Unbiased discovery of in vivo imaging probes through in vitro profiling of nanoparticle libraries.
- Detecting epidermal growth factor receptor tumor activity in vivo during cetuximab therapy of murine gliomas.
- Second harmonic generation imaging via nonlinear endomicroscopy.
- Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence.
PubMed: SPECT In Vivo Imaging
- Insertion of a lysosomal enzyme cleavage site into the sequence of a radiolabeled neuropeptide influences cell trafficking in vitro and in vivo.
- A standardised study to compare prostate cancer targeting efficacy of five radiolabelled bombesin analogues.
- Combined optical and single photon emission imaging: preliminary results.
- An Ultrahigh Resolution SPECT System for I-125 Mouse Brain Imaging Studies.
- Targeting Prostate Cancer Cells In Vivo Using a Rapidly Internalizing Novel Human Single-Chain Antibody Fragment.
PubMed: PET In Vivo Imaging
- Cerenkov radiation allows in vivo optical imaging of positron emitting radiotracers.
- Design and optimization of coin-shaped microreactor chips for PET radiopharmaceutical synthesis.
- Molecular neuroimaging in rodents: assessing receptor expression and function.
- Biodistribution and Imaging of 1-(2-Deoxy-beta-D-Ribofuranosyl)-2,4-Difluoro-5-[(123/125)I]Iodobenzene (dRF[(123/125)I]IB), A Nonpolar Thymidine-Mimetic Nucleoside, in Rats and Tumor-Bearing Mice.
- Evaluation of Bifunctional Chelates for the Development of Gallium-Based Radiopharmaceuticals.
PubMed: MRI In Vivo Imaging
- Functional L-lysine dendritic macromolecules as liver-imaging probes.
- Washout of small molecular contrast agent in carcinoma-derived experimental tumors.
- Tead-1 over-expression in the mouse heart promotes an age-dependent heart dysfunction.
- Activatable magnetic resonance imaging agents for myeloperoxidase sensing: mechanism of activation, stability, and toxicity.
- Hyperpolarized [2-13C]-fructose: a hemiketal DNP substrate for in vivo metabolic imaging.
PubMed: CT in Vivo Imaging
- Tissue-engineered vascular grafts transform into mature blood vessels via an inflammation-mediated process of vascular remodeling.
- Genetic deficiency of Syk protects mice from autoantibody-induced arthritis.
- Apoptotic analysis of memory T cells and dendritic cells during the early stages of viral infection or exposure to TLR agonists.
- Bortezomib inhibits hepatitis B virus replication in transgenic mice.
- Rapid Three-Dimensional Phenotyping of Cardiovascular Development in Mouse Embryos by Micro-CT with Iodine Staining.
PubMed: Ultrasound In Vivo Imaging
- Ultrasound-enhanced delivery of targeted echogenic liposomes in a novel ex vivo mouse aorta model.
- IFN-{beta} Restricts Tumor Growth and Sensitizes Alveolar Rhabdomyosarcoma to Ionizing Radiation.
- Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy.
- Ultrasound-Microbubble-Mediated Intercellular Adhesion Molecule-1 Small Interfering Ribonucleic Acid Transfection Attenuates Neointimal Formation After Arterial Injury in Mice.
- Uterine NK Cells, Spiral Artery Modification and the Regulation of Blood Pressure During Mouse Pregnancy.
PubMed: Intravital Microscopy In Vivo Imaging
- Prothrombotic effects of diclofenac on arteriolar platelet activation and thrombosis in vivo.
- Accelerated angiogenic host tissue response to PLGA- scaffolds by vitalization with osteoblast like cells.
- VEGF incorporated into calcium phosphate ceramics promotes vascularisation and bone formation in vivo.
- Uterine NK Cells, Spiral Artery Modification and the Regulation of Blood Pressure During Mouse Pregnancy.
- Systemic antiangiogenic activity of cationic poly-L-lysine dendrimer delays tumor growth.
