Welcome to:
The In Vivo Imaging Community
The In Vivo Imaging Page provides a Discussion Forum and Links to information about the discipline of "In Vivo" /Molecular Imaging with an emphasis on Pre-clinical Animal Models, not excluding Translational Imaging. Techniques covered are: Optical Imaging, Molecular Imaging, Fluorescence Imaging, Bioluminescence Imaging, MRI, PET, SPECT, CT, Ultrasound and Intravital Microscopy.
Sign Up to access full Resources.
In Vivo Imaging - White Papers
In Vivo Optical Imaging Reporters
There is a plethora of choice in reporter tags for non-invasive in vivo optical imaging (fluorescence and bioluminescence) that are currently available from various commercial suppliers. This generates tremendous flexibility on one hand, but dilemma of choice on the other. This manuscript reviews the determining factors and will facilitate decision making using an interactive flow chart. Read more
D-Luciferin: in vivo imaging methods
D-luciferin [(S)-2-(6′-hydroxy-2′-benzothiazolyl)thiazoline-4-carboxylic acid] is the substrate of the North American firefly Photinus Pyralis luciferase and Clickbeetle Red and Clickbeetle Green luciferase. Luciferin is a low molecular weight (318.41 g/mole) organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is a small molecule which freely diffuses across membranes. When luciferin is injected in vivo Read more
Mouse Imaging: mouse strains for optical imaging
Laboratory mice are the primary model in preclinical research. This review discusses the characteristics of mouse strains of interest for in vivo optical mouse imaging in general and for cancer models. Read more
Cell Culture Techniques for In Vivo Grafting
Mouse Microbubble Imaging – contrast enhanced ultrasound, gene therapy and drug delivery
Microbubbles for in vivo ultrasound mouse imaging are encapsulated, inert gas filled colloidal particles. Upon intravenous injection, they are used as ultrasound contrast agents. Read more
In Vivo imaging - In the News
Study finds how nanoparticles indirectly damage DNA in cells
British researchers who built a multi-layer "barrier" of human cells to replicate the function of specialized protective tissues in the body found that signals transmitted by nanoparticles through the protective barrier indirectly damage DNA inside cells. The study results "expand significantly the hurdles that any theoretical nano-safety assessment would need to clear," an expert said. The discovered mechanism, however, also opens up the possibility of being able to deliver treatments across barriers without actually crossing them, another expert suggested.
Canary Funds Early Cancer Center at Stanford
NEW YORK (GenomeWeb News) – Stanford University will use a $15 million commitment from the Canary Foundation, along with $5 million of its own funds, to start a cancer research center focused on using proteomics and imaging techniques to find ways to identify cancer earlier.
Preclinical Drug Development Must Consider the Impact on Metastasis
Drs. Steeg et al., President and members of the "Metastasis Research Society " propose that preclinical anticancer drug development be required to show efficacy in at least one metastasis model, preferably incorporating metastasis from an orthotopic site. Robust models are available with relevant histology and Imaging for quantification.
Clin Cancer Res 2009; 15 (14) July 15, 2009
Infrared-fluorescent proteins for whole body imaging
2008 Nobel-Prize winner Roger Tsien, Ph.D., has engineered infrared-fluorescent proteins (IFPs) that are are suitable for whole-body imaging in small animals.
The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008 (Jove video)
Upcoming Events
April 18-21, 2010 - Washington DC
May 17-22, 2010 - Washington DC
May 26-29, 2010 - Warsaw, Poland
SHARE your "in vivo" imaging Know How
-
How To - Help
If you need assitance, have suggestions, would like to advertise or be our "Featured Researcher", see:
- contact: support@invivoimagingcommunity.com
Recent Publications : Pubmed - Luciferase In Vivo Imaging
- Generation of a Novel Transgenic Mouse Model for Bioluminescent Monitoring of Survivin Gene Activity in Vivo at Various Pathophysiological Processes. Survivin Expression Overlaps with Stem Cell Markers.
- Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo.
- Mature and functional viral miRNAs transcribed from novel RNA polymerase III promoters.
- The cerebellum harbors a circadian oscillator involved in food anticipation.
- Icariin protects against bone loss induced by oestrogen deficiency and activates oestrogen receptor-dependent osteoblastic functions in UMR 106 cells.
PubMed: Fluorescence In Vivo Imaging
- In vivo Fluorescence Imaging of Muscle Cell Regeneration by Transplanted EGFP-labeled Myoblasts.
- In vivo imaging of ocular MCMV infection.
- In-vivo studies on intraperitoneally administrated poly(vinyl alcohol).
- A reporter gene imaging system for monitoring microRNA biogenesis.
- Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus.
PubMed: SPECT In Vivo Imaging
- Effects of Pluronic and Doxorubicin on Drug Uptake, Cellular Metabolism, Apoptosis and Tumor Inhibition in Animal Models of MDR Cancers.
- Small-animal SPECT and SPECT/CT: application in cardiovascular research.
- Quinoline-n-butylcyanoacrylate-based nanoparticles for brain targeting for the diagnosis of Alzheimer's disease.
- Synthesis and Characterization of Iodinated Tetrahydroquinolines Targeting the G Protein-Coupled Estrogen Receptor GPR30.
- Covalently combining carbon nanotubes with anticancer agent: preparation and antitumor activity.
PubMed: PET In Vivo Imaging
- The Forefront for Novel Therapeutic Agents Based on the Pathophysiology of Lower Urinary Tract Dysfunction: Bladder Selectivity Based on In Vivo Drug-Receptor Binding Characteristics of Antimuscarinic Agents for Treatment of Overactive Bladder.
- A HER2-binding Affibody molecule labelled with (68)Ga for PET imaging: direct in vivo comparison with the (111)In-labelled analogue.
- Different Strategies for Reducing Intestinal Background Radioactivity Associated with Imaging HSV1-tk Expression Using Established Radionucleoside Probes.
- Direct detection and quantification of abasic sites for in vivo studies of DNA damage and repair.
- In Vivo Evaluation of alpha7 Nicotinic Acetylcholine Receptor Agonists [C]A-582941 and [C]A-844606 in Mice and Conscious Monkeys.
PubMed: MRI In Vivo Imaging
- Methods for an improved detection of the MRI-CEST effect.
- 1H and hyperpolarized 3He magnetic resonance imaging clearly detect the preventative effect of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo.
- Molecular Magnetic Resonance Imaging of Myocardial Angiogenesis After Acute Myocardial Infarction.
- Reduced blood brain barrier breakdown in P-selectin deficient mice following transient ischemic stroke: a future therapeutic target for treatment of stroke.
- Oxazine conjugated nanoparticle detects in vivo hypochlorous acid and peroxynitrite generation.
PubMed: CT in Vivo Imaging
- Analytical radiography for planar radiographic images implemented with a multi-modal system.
- Mammalian target of rapamycin regulates vascular endothelial growth factor-dependent liver cyst growth in polycystin-2-defective mice.
- Expression of the BabA Adhesin During Experimental Infection with Helicobacter pylori.
- Therapeutic efficacy and microSPECT/CT imaging of (188)Re-DXR-liposome in a C26 murine colon carcinoma solid tumor model.
- Pro416Arg cherubism mutation in Sh3bp2 knock-in mice affects osteoblasts and alters bone mineral and matrix properties.
PubMed: Ultrasound In Vivo Imaging
- Timing of bone marrow cell delivery has minimal effects on cell viability and cardiac recovery after myocardial infarction.
- Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography.
- Mesenchymal stem cells promote matrix metalloproteinase secretion by cardiac fibroblasts and reduce cardiac ventricular fibrosis after myocardial infarction.
- Ultrasonic disruption of the blood-brain barrier enables in vivo functional mapping of the mouse barrel field cortex with manganese-enhanced MRI.
- Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo.
PubMed: Intravital Microscopy In Vivo Imaging
- Reduced blood brain barrier breakdown in P-selectin deficient mice following transient ischemic stroke: a future therapeutic target for treatment of stroke.
- Determination of Microcirculatory Changes and Angiogenesis in a Model of Frostbite Injury In Vivo.
- Intravital imaging of DSS-induced cecal mucosal damage in GFP-transgenic mice using two-photon microscopy.
- Photodynamic therapy induces selective extravasation of macromolecules: Insights using intravital microscopy.
- Exogenous Nitric Oxide Donation Causes Desensitization of Arteriolar Relaxing Activity In Vivo: An Intravital Analysis in Mice.
